Exonuclease III, 25,000 U @ 200 U/uL OverExpress C41(DE3) 003 in 200 ml of

003 in 200 ml of LB Miller medium and grown at 37° C with shaking at 210 rpm

CircLigase ssDNA Ligase ligates ends of ssDNA in the absence of a complementary sequence

Avoid T1 and T5 phage contamination with tonA mutation

BAC-Optimized Replicator Cells are components of Lucigen’s CopyRight®Cloning Kits

subcloning

Exonuclease III, 25,000 U @ 200 U/uL OverExpress C41(DE3) 003 in 200 ml ofDigest duplex DNA in a 3 to 5 direction from a nick, blunt end, or 3 recessed end, producing stretches of ssDNA. Produce stretches of ssDNA in dsDNA templates containing nicks, blunt ends or recessed 3' ends Protect protruding 3' ends becuase this exonuclease will not start digestion at protruding 3' overhangs Applications Production of intermediates for site directed mutagenesis. 1 3 Production of strand specific radiolabeled probes. 4 Exonuclease