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Treatment with 5-amino-1mq upregulates proliferationrelated genes (Ki67, PCNA), promotes cell cycle progression, and improves cell fusion efficiency to form more multinucleated myotubes, achieving functional levels similar to those of young mice.Additionally, 5-amino-1mq inhibits muscle stem cell apoptosis, reduces oxidative stress damage, and maintains stemness

Our study also suggests that upregulating the expression of DDIT4 in target cells and organs could serve as a strategy to improve AMSC therapeutic potency, which establish a theoretical foundation for the further development of stem cell-based alternative therapies

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