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Secondary Correction Phase: Weekly Injections for One Month After the first phase, the secondary correction phase starts

Plasmids and site-directed mutagenesis For the RNA interference-mediated knockdown of KEAP1, we used the lentiviral plasmid pLKO.1 from the TRC shRNA library (Sigma-Aldrich, Milan, Italy) expressing specific shRNA for 3UTR of human KEAP1 (#TRCN0000155340) or GFP as negative control [65]

Finlay, S., Rudd, D., McDermott, B

doi:10.1016/j.giec.2012.07.006 Di Sabatino A, Corazza GR

Therefore, superoxide radicals are more effective reducing (those that lose electrons) than oxidizing (those that gain electrons) agents